DNA Immunization Technique in Diseases Research

DNA immunization is a technique for protecting an organism against disease by injecting it with genetically engineered DNA to produce an immunological response. It has a number of advantages over conventional immunization, including the ability to induce a wider range of immune response types.

DNA immunization, also known as gene immunization, polynucleotide vaccine and DNA vaccine, is a newly established immunological theory and technique first discovered in the 1990s. Compared with current protein vaccine, DNA immunization is safer and more inexpensive. Besides, it induces a more effective immune response and is easy to be prepared. With the potential application in some specific field, DNA immunization is not only been widely used in anti-virus, bacteria, fungi, parasites as well as other anti-infection immunity, but also plays a n important role in tumor immunology and autoimmune disease. Like all other groups, Creative Biolabs follows up with the updated technique in DNA immunization, and made their own innovation by putting DNA immunization in the production of antibody.

Creative BioLabs has developed Direct Antibody Technology™ (DAT) to produce custom polyclonal and monoclonal antibodies. As a highly optimized service, this DNA immunization technology relies on a proprietary genetic immunization procedure using plasmid DNA encoding the target protein of interest. The immunized hosts then produce the encoded protein and raise antibodies in vivo.

To ensure the accomplishment of the genetic immunization, the cDNA-encoded protein must be secreted by the transfected cells in immunized animals or expressed on the surface of the transfected cells. Creative BioLabs introduces the gene in the form of a cDNA directly into an animal, which translates this cDNA into protein thus stimulating an immune response against the foreign protein. As a result, synthesis and purification of protein immunogens is not necessary for this genetic immunization approach.

Creative Biolabs’ Direct Antibody Technology™ maximizes the likelihood of producing and maintaining the native structure of the antigen. The foremost advantage of this antibody production approach is its high success rate in generation of high-affinity antibodies recognizing membrane proteins (such as 7-membrane-spanning GPCR proteins, ion channels and other multiple membrane spanning proteins) in their native conformation, unknown proteins whose genes have been obtained, toxic proteins, insoluble proteins, proteins containing disulfide bonds, post-translational modified proteins, or large protein domains. For therapeutic antibodies, however, the antigens in their native conformation should be targeted with a high-affinity.

Creative Biolabs is a leading service provider in the field of DNA immunization. 

Creative Biolabs released membrane protein expression service

Creative Biolabs released cell-free membrane protein expression service for protein related research.

Membrane proteins occupy one fourth of total encoding genes in genome. They localize on the cell membrane, play various roles such as signal transduction, transportation and ligand-binding. It is estimated more than half of the drug candidates target membrane proteins, mainly G-protein coupled receptors (GPCRs) and ion channels. However, due to the nature of membrane protein, traditional protein expression methods encounter obstacles such as low amount of expression, inadequate post-translational modification, misfolding, and loss of bio-activity.

Aiming to solve the problems in the conventional methods, Creative Biolabs designs a novel cell-free membrane protein expression system. In this unique system, the neonatal membrane protein inserts into liposome, which is well designed to imitate the in vivo membrane features, keeping the native conformation and bio-activity to the greatest extent.

The cell-free expression system also possesses other advantages: high purity of target protein, rapid and stable expression, high yield and good repeatability. The system is also easy to be adapted to diverse requirement from our clients, such as the size and modification of liposome, the therapeutics encapsuled in the liposome and type of solvent reagents. Our system is tailored for customers in membrane protein projects of antibody screening, ligand discovery, drug delivery, vaccine development, and structural determination.

About Creative Biolabs

Creative Biolabs is specialized in providing custom biotechnology and pharmaceutical services that cover the full scope of biotechnology needs of early drug discovery and drug development. As a trusted provider of the most cost-effective outsourcing solutions, Creative Biolabs has been working for a large number of satisfied clients from biotechnology and pharmaceutical companies as well as government and academic research laboratories all over the world. It was founded by scientists who are dedicated to the conquering of cancer. We believe to build up a custom-service-centered business model is important for optimizing the drug development process, leveraging accessible resources, and forming a team of various background to conduct drug discovery in future. Our commitment to this long-term goal motivates us to deliver the highest quality results to our clients with speed.